Serena and Venus Williams suffered a 7-6 (5), 6-4 loss to Czech pair Lucie Hradecka and Linda Noskova in the first round of US Open doubles Thursday night at Arthur Ashe Stadium.
It features two American sisters who have won 14 Grand Slam doubles titles but are partnering for the first time since the 2018 French Open.
This was their fourth loss in the first round of doubles at a Slam; The most recent was at the 2013 French Open.
After the match, the sisters hugged each other. He left the court standing up.
A sold-out crowd of 23,859 was seen in each of Serena's two singles wins so far this week, although the fans weren't as enthusiastic.
On Thursday it was for those other night matches that included a player who indicated it would be the final tournament of her career. Venus lost in the first round of singles.